Chromatin from activated SCs were isolated and sonicated to 100-300nt for immunoprecipitation with antibodies. Purified RNAs were fragmented and the cDNA were synthesized by reverse transcription. The resulting double-stranded DNA fragments were end-repaired and A-nucleotide overhangs were added. ChIP-seq and RNA-seq libraries were constructed according to the standard illumina pair-end sequencing procedure.