GSM2891936: Pol II NHS replicate 1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Embryonic fibroblast
Cell type
NIH/3T3
Primary Tissue
Embryo
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
embryonic fibroblast
cell line
NIH 3T3 fibroblast cell line
treatment
control
chip antibody
D8L4Y, Cell Signalling Technology
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sheared chromatin was prepared by sonicating nuclei, Pol II-DNA complexes were immunoprecipitated. Libraries were made using the Ovation Ultralow System V2 from NuGEN. Libraries were sequenced on an Illumina HiSEQ 4000