Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Embryo

Cell type

Cochlea

MeSH Description

The part of the inner ear (LABYRINTH) that is concerned with hearing. It forms the anterior part of the labyrinth, as a snail-like structure that is situated almost horizontally anterior to the VESTIBULAR LABYRINTH.

Sample

source_name

Mouse cochlea

strain

C57BL/6

developmental stage

E13.5

tissue

cochlea

antibody

None

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The tissues were fixed with 1%PFA in PBS for 30 min at RT. After washing, the tissues were homogenized to a fine powder using BeadBugTM high-energy benchtop homogenizer in the lysis buffer. Chromatin shearing was performed using the Covaris E220 focused-ultrasonicator system. After preclearing with Protein A beads, the chromatin was immunoprecipitated with antibodies. After IP, the beads were washed in sequential low salt buffer, high salt buffer, LiCl washing buffer and TE buffer. The libraries were prepared using the ThruPlex DNA-seq Kit (Rubicon Genomics) and according to the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

42853510

Reads aligned (%)

97.3

Duplicates removed (%)

4.5

Number of peaks

485 (qval < 1E-05)

mm9

Number of total reads

42853510

Reads aligned (%)

97.2

Duplicates removed (%)

4.5

Number of peaks

476 (qval < 1E-05)