GSM2888537: NK D0 3 [ATAC-seq]; Mus musculus; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
Natural killer cells
NA
NA
Attributes by original data submitter
Sample
source_name
splenic NK cells
genotype
WT
replicate
3
ly49expression
HPOS
condition
MCMVD0
cell type
splenic NK cells
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Lymphocytes were harvested as described in manuscript. Fresh cells were prepared as previously described (Buenrostro et al., 2013). Briefly, cells were washed in cold PBS and lysed. Transposition occurred at 42°C for 45 minutes. DNA was purified using the MinElute PCR purification kit (Qiagen) and amplified for 5 cycles. Additional PCR cycles were evaluated by real time PCR. Final product was cleaned by Ampure Beads at a 1.5x ratio. Libraries were sequenced on a Hiseq 2500 1T in a 50bp/50bp paired-end run, using the TruSeq SBS Kit v3 (Illumina). An average of 51 million paired reads were generated per sample.