Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Others

Cell type

Aska

NA

NA

Sample

source_name

Aska (SS)

cell line

Aska (SS)

shRNA

shSSX

antibody

anti-H3K27me3

antibody vendor

Millipore

antibody catalog

07-449

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation (ChIP) experiments were performed per standard protocols (Millipore, Billerica, MA) with minor modifications. Briefly, 10M cells were crosslinked with 1% formaldehyde for 10 min at 37 °C. This reaction was subsequently quenched with 0.125M glycine. All library prep and sequencing were 75bp single end on Illuminia Nextseq 500

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

25394121

Reads aligned (%)

97.8

Duplicates removed (%)

9.7

Number of peaks

272 (qval < 1E-05)

hg19

Number of total reads

25394121

Reads aligned (%)

97.4

Duplicates removed (%)

10.1

Number of peaks

284 (qval < 1E-05)