Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived cardiac cells
NA
NA

Attributes by original data submitter

Sample

source_name
Cardiac progenitor stage cells differentiated from H7
cell type
Cardiac progenitor stage cells differentiated from H7
chip antibody vender
Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
(RNA-seq) iPSC-CMs were detached from cell culture plates and RNA was extracted using a microRNeasy kit (QIAGEN). (ChIP-seq) Lysates were clarified from sonicated nuclei and Protein-DNA complexes were isolated with antibody. (RNA-seq) RNA libraries were prepared for sequencing using standard Illumina protocols. (ChIP-seq) ChIP-seq libraries were prepared for sequencing using The Ion Proton™ System protocols or NEBNext DNA Library Prep Master Mix Set for Illumina kit (E6040S).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
34093562
Reads aligned (%)
97.2
Duplicates removed (%)
2.1
Number of peaks
1135 (qval < 1E-05)

hg19

Number of total reads
34093562
Reads aligned (%)
95.9
Duplicates removed (%)
2.2
Number of peaks
675 (qval < 1E-05)

Base call quality data from DBCLS SRA