GSM2877299: H3K27Ac S427F Rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Others
Cell type
JMSU1
Primary Tissue
Bladder
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
bladder cancer cells
cell type
JMSU-1
chip antibody
H3K27Ac (Abcam, 4729, lot GR286678-2)
genotype
RXRA S427F
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinking and ChIP were performed as described for adherent cells in the ENCODE experiment summary for ENCSR000BJW (https://www.encodeproject.org/experiments/ENCSR000BJW/) . Chromatin was sonicated using the Diagenode Pico Bioruptor. DNA-protein complexes were precipitated with antibodies against RXRA (PP-K8508-00 R&D Systems) and H3K27Ac (ab4729 Abcam).