ChIP-Atlas: SRX3447948

Antigen

Cell type Class: Neural
Cell type: Microglia
MeSH Description: The third type of glial cell, along with astrocytes and oligodendrocytes (which together form the macroglia). Microglia vary in appearance depending on developmental stage, functional state, and anatomical location; subtype terms include ramified, perivascular, ameboid, resting, and activated. Microglia clearly are capable of phagocytosis and play an important role in a wide spectrum of neuropathologies. They have also been suggested to act in several other roles including in secretion (e.g., of cytokines and neural growth factors), in immunological processing (e.g., antigen presentation), and in central nervous system development and remodeling.

source_name: FACS-purified microglia from brains of embryos of GF and SPF dams (E14.5)
developmental stage: E14.5
Sex: not determined
conditions: GF
tissue: whole brain
strain: C57BL/6J

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: Sorted cells were resuspended in Lysis buffer (10mM Tris-HCl pH 7.4, 10mM MgCl2, 0.1% IGEPAL CA-630) and underwent Transposase reaction with Tn5 For library amplification, two sequential 9-cycle PCR were performed in order to enrich small tagmented DNA fragments. 2μl of indexing primers included in the Nextera Index kit and KAPA HiFi HotStart ready mix were used, then after the first PCR, the libraries were selected for small fragments (less than 600 bp) using SPRI cleanup. A second PCR was performed with the same conditions in order to obtain the final library. DNA concentration was measured with a Qubit fluorometer (Life Technologies) and library sizes were determined using TapeStation (Agilent Technologies). Libraries where sequenced on a NextSeq for an average of 10 million unique reads per sample.