Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TAL1

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
Human K562 cells
cell line
K562
genotype/variation
MYO1D Hub KO
chip antibody
Santa Cruz Biotechnology,sc-12984,C1315

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq analysis using the Illumina NextSeq500, 1-10 ng of ChIP DNA was processed for library generation using the NEBNext Ultra II DNA Library Prep kit following the manufacturer's protocol (New England Biolabs). Raw reads that aligned to exactly one location in the reference human genome (hg19) were retained for downstream data analysis.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
12508837
Reads aligned (%)
90.7
Duplicates removed (%)
6.8
Number of peaks
8606 (qval < 1E-05)

hg38

Number of total reads
12508837
Reads aligned (%)
92.2
Duplicates removed (%)
6.0
Number of peaks
8633 (qval < 1E-05)

Base call quality data from DBCLS SRA