Antigen

Antigen Class

TFs and others

Antigen

Mecp2

Cell type

Cell type Class

Neural

Cell type

Frontal cortex

NA

NA

Sample

source_name

Frontal cortex

strain

129/FVB

genotype/variation

WT

chip antibody

MeCP2 (Millipore, #17-10491)

tissue

Frontal cortex

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclear lysates were sonicated and MeCP2-DNA complexes were isolated with Anti-MeCP2 antibody. Library was prepared by Genomic and RNA Profiling Core at Baylor College of Medicine using ThruPLEX DNA-seq kit (Rubicon).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

117093937

Reads aligned (%)

97.8

Duplicates removed (%)

18.9

Number of peaks

794 (qval < 1E-05)

mm9

Number of total reads

117093937

Reads aligned (%)

97.7

Duplicates removed (%)

19.1

Number of peaks

773 (qval < 1E-05)