Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mouse embryonic stem cell

chip antibody

H3K27ac

genotype

MLL3-/-;MLL4flox/flox

cell type

embryonic stem cell

genetic background

C57BL/6 and SV/129

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-Seq, suspended 10 x 106 ESCs (feeder removed) and EBs are fixed with 1% formaldehyde in culture medium, followed by ChIP assay. For RNA-Seq, total RNA are collected using Trizol extraction followed by mRNA purification using Dynabeads® mRNA DIRECT™ Kit (invitrogen). Libraries were prepared according to Illumina TruSeq RNA and DNA sample preparation Kit

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

19178218

Reads aligned (%)

95.0

Duplicates removed (%)

6.1

Number of peaks

9033 (qval < 1E-05)

mm9

Number of total reads

19178218

Reads aligned (%)

94.9

Duplicates removed (%)

6.2

Number of peaks

8992 (qval < 1E-05)