Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
antibody
11966
supplier
Cell Signaling
lot
Lot 2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 0.3% formaldehyde for 30 minutes at 4 degrees prior to processing for ChIP using protocol from Raab et al 2015. Kapa DNA hyperpep kit

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
32534863
Reads aligned (%)
91.2
Duplicates removed (%)
0.7
Number of peaks
2833 (qval < 1E-05)

hg19

Number of total reads
32534863
Reads aligned (%)
90.4
Duplicates removed (%)
0.6
Number of peaks
2651 (qval < 1E-05)

Base call quality data from DBCLS SRA