Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

AOS-14_E-n

strain background

C57BL/6

cell line

AOS-14

cell type

mouse haploid embryonic stem cells

chip antibody

H3K27ac (Abcam, ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from nuclei and then fragmented with Mnase treatment. Histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit

Sequencing Platform

instrument_model

HiSeq X Ten

mm10

Number of total reads

18400096

Reads aligned (%)

92.4

Duplicates removed (%)

12.0

Number of peaks

135 (qval < 1E-05)

mm9

Number of total reads

18400096

Reads aligned (%)

92.3

Duplicates removed (%)

12.0

Number of peaks

106 (qval < 1E-05)