Stable and unique insertion of a 5xUAS followed by Luciferase sequence in the first intron of NCOA5 (Stielow et al. EMBO Rep. 2008)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% formaldehyde for 15 minutes before lysis and chromatin extraction. ChIP were performed overnight using Dynabeads (ThermoFisher Scientific) coupled with the appropriate antibodies. Beads were washed and crosslinking was reversed by heating samples at 65˚C overnight. Recovered DNA was subsequently purified with MinElute PCR Purification Kit (Qiagen) according to the manufacturer's protocol. ChIP-Seq_NEB ultraII according to manufacturer's instructions