GSM2864779: Non-immune IgGs; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA
Attributes by original data submitter
Sample
source_name
human dermal fibroblasts (HDFs)
chip antibody
Non-immune IgGs
cell type
dermal fibroblast
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immune-precipitation was carried from 15 × 10^6 HDFs as for the ChIP assay with anti-AR antibody versus nonimmune IgGs. The bead-bound chromatin was tagged with Tn5 transposase (Nextera DNA Sample Prep kit, Illumina). The “tagmented” chromatin was de-cross-linked, purified, and amplified (20 cycles) using tag-specific primers. The amplified products were subjected to size selection (200-400 bp) and sequenced using Illumina Hi-seq.