Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Digestive tract

Cell type

Intestines

MeSH Description

The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.

Sample

source_name

intestinal epithelial cells

strain

C57B6

tissue

large intestine

cell type

IEC

antibody

na

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

10 μg of chromatin was incubated with anti-H3K9Ac antibody. Protein A sepharose beads were used to obtain the ChIP product. Chromatin was reverse crosslinked at 65℃ 16hours, and DNA was purified by phenol/chloroform extraction. Intestinal epithelial cells from the large intestine were fixed in 1% PFA for 10 min and quenched with glycine. Cells were lysed and extracts were sonicated using a Bioruptor (Diagenode) and appropriate sonication was confirmed using an Agilent bioanalyzer. ChIP and input DNA libraries for each genotype were prepared for sequencing according to the multiplex amplification protocol from Illumina.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

24636812

Reads aligned (%)

97.5

Duplicates removed (%)

14.5

Number of peaks

485 (qval < 1E-05)

mm9

Number of total reads

24636812

Reads aligned (%)

97.2

Duplicates removed (%)

14.5

Number of peaks

533 (qval < 1E-05)