GSM1218887: input DNA D0 MLL4 KO; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Adipocyte
Cell type
Brown preadipocytes
NA
NA
Attributes by original data submitter
Sample
source_name
mouse brown preadipocytes, MLL4 KO, input
strain/background
C57BL/6J
genotype/variation
MLL3-/-;MLL4-/-
age
E18.5 embryos
cell type
immortalized brown preadipocytes
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei, and histone-DNA complexes were isolated with antibody (H3K4me1: abcam, ab8895 (lot: GR61280-1); H3K4me2: abcam, ab7766 (lot: GR102810-1); H3K4me3: Millipore, 17-614 (lot: 2109590); H3K27ac: abcam, ab4729 (lot: GR81163-1)). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit.