Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

Megakaryocyte-erythroid progenitor

NA

NA

Sample

source_name

Bone Marrow

tissue

MEPs

genotype

Mof -/+

strain

C57BL/6

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The library was completed and amplified through a PCR following the DeepSeq using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina default instructions (NEB #E7645). ChIP libraries were sequenced using an Illumina HiSeq 3000.

Sequencing Platform

instrument_model

Illumina HiSeq 3000

mm10

Number of total reads

31148937

Reads aligned (%)

69.1

Duplicates removed (%)

82.5

Number of peaks

131 (qval < 1E-05)

mm9

Number of total reads

31148937

Reads aligned (%)

69.0

Duplicates removed (%)

82.5

Number of peaks

100 (qval < 1E-05)