GSM2861545: KELLY CYC065 H3K27AC; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Neural
Cell type
KELLY
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma
Attributes by original data submitter
Sample
source_name
Chromatin IP against H3K27ac in CYC065 KELLY
cell line
KELLY
cell type
Neuroblastoma
input
KELLY_CYC065_WCE
treatment
1uM CYC065 for 3HR
growth protocol
10% FBS, DMEM
barcode
TTGACCCT
antibody
H2K27ac
antibody maker
abcam
antibody catalog number
ab4729
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using Tn5 enzyme and amplified using 2x NEBNext HiFi master mix. ChIPmentation (Schmidl et a., 2015; PMC4589892)