Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Liver

Cell type

Hepa 1-6

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

cells were cultured in standard conditions before fixation

tissue

Hepa 1.6 hepatoma cell line

antibody

H3K9me3 (Diagenode, C15410056)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP and library preparation were done according to (Ecco, Cassano et al. 2016), with modifications as described in Supplemental Experimental Procedures. Libraries were ligated with Illumina adaptors

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

115478856

Reads aligned (%)

90.1

Duplicates removed (%)

37.6

Number of peaks

1513 (qval < 1E-05)

mm9

Number of total reads

115478856

Reads aligned (%)

89.8

Duplicates removed (%)

37.5

Number of peaks

1658 (qval < 1E-05)