Antigen

Antigen Class

TFs and others

Antigen

Kmt2d

Cell type

Cell type Class

Adipocyte

Cell type

Brown preadipocytes

NA

NA

Sample

source_name

mouse brown preadipocytes, MLL4 KO, MLL4 ChIP

strain/background

C57BL/6J

genotype/variation

MLL3-/-;MLL4-/-

age

E18.5 embryos

cell type

immortalized brown preadipocytes

chip antibody

MLL4

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei, and MLL4-DNA complexes were isolated with homemade MLL4 antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

30889355

Reads aligned (%)

75.9

Duplicates removed (%)

26.1

Number of peaks

17209 (qval < 1E-05)

mm9

Number of total reads

30889355

Reads aligned (%)

75.7

Duplicates removed (%)

26.2

Number of peaks

17034 (qval < 1E-05)