Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Digestive tract

Cell type

SW 480

Primary Tissue

unresolved

Tissue Diagnosis

unresolved

Sample

source_name

SW480 cell line

chip antibody

H3K4me2 (Millipore Cat. No. 07-030)

tumor stage

Dukes' type B, colorectal adenocarcinoma

treatment

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-sequencing libraries were prepared by using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB Cat. No. E7370), according to the manufacturer's instructions. The sequencing was performed by Hiseq1500 (Illumina).

Sequencing Platform

instrument_model

Illumina HiSeq 1500

hg38

Number of total reads

23489301

Reads aligned (%)

98.3

Duplicates removed (%)

5.8

Number of peaks

46402 (qval < 1E-05)

hg19

Number of total reads

23489301

Reads aligned (%)

97.8

Duplicates removed (%)

6.6

Number of peaks

46383 (qval < 1E-05)