Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Cell line

Cell type

S2R+

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

ChIP Seq of Pol II in Btz KD

cell line

S2R+

cell type

Embryonic

pulldown

RBP1 (Diagenode Cat No-15200004)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was recovered after reverse crosslinking and phenol chloroform extraction. After precipitating and pelleting, DNA was dissolved in 30 _l of TE After checking enrichment the recovered DNA was converted into libraries using NebNext Ultra DNA library preperation kit, following manufacturer's protocol NebNext UltraII

Sequencing Platform

instrument_model

NextSeq 500

dm6

Number of total reads

21522145

Reads aligned (%)

84.8

Duplicates removed (%)

9.5

Number of peaks

9457 (qval < 1E-05)

dm3

Number of total reads

21522145

Reads aligned (%)

84.9

Duplicates removed (%)

8.7

Number of peaks

10189 (qval < 1E-05)