Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Blood
Cell type
Leukocyte stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
bone marrow induced leukocyte stem
strain
C57BL/6
chip antibody
H3K27me3 antibody (#07-449, Millipore)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Genomic DNA was isolated using QIAGEN All-prep kit. Library was prepared according to Illumina TruSeq protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 1500

mm10

Number of total reads
35249607
Reads aligned (%)
96.2
Duplicates removed (%)
41.9
Number of peaks
11930 (qval < 1E-05)

mm9

Number of total reads
35249607
Reads aligned (%)
95.6
Duplicates removed (%)
41.8
Number of peaks
11947 (qval < 1E-05)

Base call quality data from DBCLS SRA