Antigen

Antigen Class

TFs and others

Antigen

Spi1

Cell type

Cell type Class

Blood

Cell type

Dendritic Cells

MeSH Description

ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Sample

source_name

dendritic cells from the spleen

cell type

CD8-positive dendritic cell line

stimulation

none

chip antibody

PU.1 (sc-352)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed as previously described (Daniel et al. Genes Dev. 2014) Indexed cDNA libraries were prepared from 1-10 ng genomic DNA using TruSeq ChIP Sample Preparation kit (Illumina).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

14264726

Reads aligned (%)

91.9

Duplicates removed (%)

27.6

Number of peaks

12660 (qval < 1E-05)

mm9

Number of total reads

14264726

Reads aligned (%)

91.6

Duplicates removed (%)

27.7

Number of peaks

12649 (qval < 1E-05)