Antigen

Antigen Class

TFs and others

Antigen

Irf5

Cell type

Cell type Class

Blood

Cell type

Dendritic Cells

MeSH Description

ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Sample

source_name

dendritic cells from the spleen

cell type

CD8-positive dendritic cell line

stimulation

90 min; 1 mM class B CpG oligonucleotide (1826)

chip antibody

IRF5 (Abcam ab21689)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed as previously described (Daniel et al. Genes Dev. 2014) Indexed cDNA libraries were prepared from 1-10 ng genomic DNA using TruSeq ChIP Sample Preparation kit (Illumina).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

81327010

Reads aligned (%)

95.2

Duplicates removed (%)

22.4

Number of peaks

28555 (qval < 1E-05)

mm9

Number of total reads

81327010

Reads aligned (%)

95.0

Duplicates removed (%)

22.4

Number of peaks

28621 (qval < 1E-05)