Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Rel

Cell type

Cell type Class
Blood
Cell type
Dendritic Cells
MeSH Description
ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Attributes by original data submitter

Sample

source_name
dendritic cells from the spleen
cell type
CD8-positive dendritic cell line
stimulation
none
chip antibody
cREL (sc-71x)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as previously described (Daniel et al. Genes Dev. 2014) Indexed cDNA libraries were prepared from 1-10 ng genomic DNA using TruSeq ChIP Sample Preparation kit (Illumina).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
22245141
Reads aligned (%)
98.0
Duplicates removed (%)
17.1
Number of peaks
305 (qval < 1E-05)

mm9

Number of total reads
22245141
Reads aligned (%)
97.8
Duplicates removed (%)
17.1
Number of peaks
361 (qval < 1E-05)

Base call quality data from DBCLS SRA