GSM2849217: E17hGFAPSrg170H3K4me2 K 1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me2
Cell type
Cell type Class
Embryo
Cell type
Embryonic brains
NA
NA
Attributes by original data submitter
Sample
source_name
embryonic cortex
age
E17.5
strain
C57BL/6J
genotype/variation
BAF dcKO_hGFAP-Cre
chip antibody
H3K4me2 (Millipore 07-449)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was isolated using Rneasy kit from Qiagen. Chromatin immunoprecipitation was performed using home-made protocol. Libraries for ChIP-Seq were prepared using NEBNext Ultra DNA Library Preparation Kit for Illumina. Libraries for RNA-Seq were prepared using Illumina TruSeq RNA Sample Preparation v2 Kit.