GSM2844016: G48-siSCR ATAC-seq; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Digestive tract
Cell type
GIST48
NA
NA
Attributes by original data submitter
Sample
source_name
G48-siSCR
cell line
GIST48
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
cell nuclei were prepared from 50,000 cells, and incubated with 2.5 μl of transposase (Illumina) in a 50-μl reaction for 30 min at 37 °C. ATAC libraries were prepared for sequencing using standard Illumina protocols, After purification of transposase-fragmented DNA, the library was amplified by PCR and subjected to paired-end 50 base-pair high-throughput sequencing on an Illumina HiSeq2500 platform.