GSM2842799: Normal Human Melanocytes Input-XL [NHM1]; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Epidermis
Cell type
Foreskin
MeSH Description
The double-layered skin fold that covers the GLANS PENIS, the head of the penis.
Attributes by original data submitter
Sample
source_name
Foreskin
cell type
foreskin, NHM1
passage
8
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For FOSL2 and TEAD4, 10x10^6 cells, were crosslinked with 0.25M DSG for 45min, followed by 1% PFA crosslinking for 10 min at RT. ChIP was performed essentially as previously described (Tian et al., Two-step Crosslinking for Analysis of Protein-chroamtin Interactions. Methods Mol Biol, 2012. 809:105-120. Roe, J.S., et al., BET Bromodomain Inhibition Suppresses the Function of Hematopoietic Transcription Factors in Acute Myeloid Leukemia. Mol Cell, 2015. 58(6): p. 1028-39). Libraries for ChIP-seq were done as previously described (Hasson D., et al., The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013 Jun;20(6):687-95).