Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Digestive tract
Cell type
KYSE-140
Primary Tissue
Esophagus
Tissue Diagnosis
Carcinoma Squamous Cell

Attributes by original data submitter

Sample

source_name
KYSE140
cell line
KYSE140
cell type
Esophageal squamous carcinoma cells
chip antibody
H3K27Ac (Abcam, ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Standard Illumina library preparation protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
39380078
Reads aligned (%)
99.0
Duplicates removed (%)
10.8
Number of peaks
19891 (qval < 1E-05)

hg19

Number of total reads
39380078
Reads aligned (%)
98.4
Duplicates removed (%)
11.8
Number of peaks
19891 (qval < 1E-05)

Base call quality data from DBCLS SRA