GSM2839022: ChIPseq H3K4me3 Day 0 2; Rattus norvegicus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Neural
Cell type
Serotonergic cells
NA
NA
Attributes by original data submitter
Sample
source_name
Serotonergic cells
cell/region
RN46A-B14 cells
chip antibody
H3K4me3 (Abcam ab8580; Lot#GR273043-6)
differentiation status
Undifferentiated
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RN46A-B14 cell pellets (10 x 106/n) and DRN tissues were crosslinked and quenched. Samples were washed thoroughly before being subjected to lysis and sonication. Samples were then incubated with specific antibodies (7.5 μg/sample) bound to M-280 Dynabeads on a rotator at 4°C overnight. The following day, immunoprecipitates were washed, eluted and reverse crosslinked. Following RNA and protein digestion, DNA fragments were purified using a Qiagen PCR purification kit. RNA and ChIP libraries were prepared for sequencing using standard Illumina protocols