Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Neural

Cell type

T98G

Primary Tissue

Brain

Tissue Diagnosis

Glioblastoma

Sample

source_name

ATAC-seq with synchronized M-phase T98G cells, rep3

cell cycle stage

Mitosis

cell line

T98G

cell type

glioblastoma

platform

HiSeq

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

For input and chromatin fractions, nuclear prep was carried out followed by sonication to solubilize chromatin. Crosslinks were reversed overnight at 65⁰ and DNA was was isolated by phenol/chloroform followed by ethanol precipitation ATAC-seq libraries were prepared as described in Buenrostro 2015. Input and chromatin fractions were prepared using the NEBnext kit as per manufacturers intstructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

58004641

Reads aligned (%)

90.6

Duplicates removed (%)

59.9

Number of peaks

4015 (qval < 1E-05)

hg19

Number of total reads

58004641

Reads aligned (%)

90.2

Duplicates removed (%)

60.3

Number of peaks

3948 (qval < 1E-05)