Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

CD4+ T cells

NA

NA

Sample

source_name

CD4+ T cells (Th17 EAE model)

background strain

C57BL6

genotype

c-Maf flfl CD4Cre

tissue

CNS (brain and spinal cord)

cell type

CD4+ T cell

time point

day 14-16 post EAE induction

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Cells sorting was followed by a transposition reaction and purification, prepared as in Buenrostro et al. Curr Protoc Mol Biol. 2015 with the transposition reaction carried out for 1h and 30 minutes at 37 °C and subsequent PCR amplification for 12 cycles

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

171694244

Reads aligned (%)

72.6

Duplicates removed (%)

39.6

Number of peaks

17025 (qval < 1E-05)

mm9

Number of total reads

171694244

Reads aligned (%)

72.5

Duplicates removed (%)

39.6

Number of peaks

17005 (qval < 1E-05)