Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

primary mouse embryonic fibroblasts

tissue

primary mouse embryonic fibroblasts

genotype

Rosa26ERT2-cre/+

antibody

RNA Pol II (4H8)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIPseq and ChIP-qPCR were performed as described without modifications (Pavri et al., 2010). In short, 20x10^6 cells were crosslinked with 1% HCHO for 10 min at 37 degree. After quenching with 0,125 M glycine, cells were sonicated with the Diagenode Bioruptor sonication machine. After chromatin-immunprecipitation, samples were end-repaired with the Epicenter End-Repair Kit. After A-tailing the samples with Klenow (exo-) enzyme, NEXTflex adapters were ligated to the samples and then purified with AMPure XP beads. PCR amplification of the libraries was performed with the Phusion-HF enzyme and the NEXTflex primer mix.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

57218281

Reads aligned (%)

85.1

Duplicates removed (%)

15.9

Number of peaks

28728 (qval < 1E-05)

mm9

Number of total reads

57218281

Reads aligned (%)

85.0

Duplicates removed (%)

15.9

Number of peaks

28701 (qval < 1E-05)