Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ONECUT2

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNCaP-Flag_OC2-ChIP-Seq-Hypoxia
tumor type
prostate cancer
cell line
LNCaP
antibody
Flag_OC2
protocol
Hypoxia

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. ChIP-enriched DNA was used for library creation with the Rubicon ThruPLEX-FD kit and sequenced (75 bp single-end reads).

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
73173901
Reads aligned (%)
18.6
Duplicates removed (%)
79.9
Number of peaks
34949 (qval < 1E-05)

hg38

Number of total reads
73173901
Reads aligned (%)
19.2
Duplicates removed (%)
79.4
Number of peaks
35279 (qval < 1E-05)

Base call quality data from DBCLS SRA