Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Blood

Cell type

Treg

NA

NA

Sample

source_name

KAP1 deficient Treg

strain

C57BL/6

tissue

Spleen

antibody

H3K9me3(abcam, ab8898, lot GR250850-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclear lysates were sonicated and histone-DNA complexes were isolated with antibody. Libraries were prepared by using DNA SMART ChIP-Seq kit (Clontech) according to the protocol. 150-400bp products were selected and subjected to the analysis.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

39398494

Reads aligned (%)

43.7

Duplicates removed (%)

47.2

Number of peaks

296 (qval < 1E-05)

mm9

Number of total reads

39398494

Reads aligned (%)

43.3

Duplicates removed (%)

46.9

Number of peaks

595 (qval < 1E-05)