GSM2818527: LNCaP shCON CDT Rep 2; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
LNCaP Cell Line
treatment
72 hour CDT
knockdown
shCON
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
ATAC-Seq was performed as previously described (Buenrostro et al 2015). Briefly, cells plated and treated as indicated by the condition. Next, 75,000 cells were collected and lysed in ATAC lysis buffer. Cells were then re-suspended in the transposition reaction mix, and the transposition reaction incubated at 37oC for 30 minutes. Transposed DNA was purified using AMPure purification methods as specified by the manufacturer. Purified DNA was then amplified utilizing custom barcoded Nextera primers as (previously listed and available in (Buenrostro et al 2015)). Library size and quality was validated using a Bioanalyzer and libraries sequenced on a NextSeq 500