Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Adult

Cell type

Ovary

NA

NA

Sample

source_name

lacI-HP1a_ovary

genotype/variation

uas-lacI-HP1a;nosGAL4/lacO-GFP

age

adult stage 5-7 days old

tissue

ovary

chip antibody

H3K4me2 (Abcam, ab7766)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Drosophila DamID was carried out according to iDamIDseq based on a method from Gutierrez-Triana JA et al.2016 Libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm6

Number of total reads

42624200

Reads aligned (%)

89.4

Duplicates removed (%)

50.7

Number of peaks

8988 (qval < 1E-05)

dm3

Number of total reads

42624200

Reads aligned (%)

89.5

Duplicates removed (%)

50.3

Number of peaks

9233 (qval < 1E-05)