Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver

strain

C57BL/6J

tissue

Liver

chip antibody

H3K27ac, Abcam, ab4729

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked sonicated chromatin was IP'ed using antibodies against H3K27Ac and DNA was extracted by phenol/choloroform. H3K27ac ChIP-seq libraries were constructed using the NEBNext Library prep kit according to manufacturer´s (NEB) instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

16812075

Reads aligned (%)

99.1

Duplicates removed (%)

44.8

Number of peaks

12404 (qval < 1E-05)

mm9

Number of total reads

16812075

Reads aligned (%)

99.0

Duplicates removed (%)

44.9

Number of peaks

12388 (qval < 1E-05)