Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
cic

Cell type

Cell type Class
Embryo
Cell type
2-4h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
2-4h gd7 embryo
genotype/variation
gd7
tissue
embryo
chip antibody
anti-cic rabbit polyclonal, custom (GenScript)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq libraries were constructed following He et al. (2011) ChIP-seq and ChIP-nexus was performed according to He et al. (2011) and He et al. (2015) with whole cell extract (WCE) derived from 100 mg embryos per ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
22465717
Reads aligned (%)
63.7
Duplicates removed (%)
33.6
Number of peaks
4484 (qval < 1E-05)

dm6

Number of total reads
22465717
Reads aligned (%)
56.5
Duplicates removed (%)
39.8
Number of peaks
4588 (qval < 1E-05)

Base call quality data from DBCLS SRA