ChIP-Atlas: SRX3268788

Antigen

Cell type Class: Neural
Cell type: Hippocampus
MeSH Description: A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Hippocampus was rapidly dissected from mice on ice, and crosslinked by 2 mM DSG for 20 min, followed by the addition of 1.5% formaldehyde during the last 10 min of DSG crosslinking. Nuclei preparation and chromatin digestion steps followed SimpleChIP Plus Enzymatic Chromatin IP Kit protocol (Cell Signaling). Chromatin was digested to 100 to 500bp fragments using MNase (micrococcal nuclease) and was immunoprecipitated with 10μg anti-L3mbtl1 antibody. ChIP-seq library barcode: ACTTGA (lane1). Libraries were prepared from 10ng ChIP'd DNA using NEBNext Ultra DNA Library Prep Kit. Size selection was performed before amplification. Final approximate fragment length is 165bp.