Antigen

Antigen Class

TFs and others

Antigen

NUP98

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC HUES6

NA

NA

Sample

source_name

HUES6, Nup98 ChIP

cell line

HUES6

cell type

embryonic stem (ES) cells

chip antibody

Nup98 (Cell Signaling #2292 )

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed in 1% formaldehyde (Polysciences) for 10min. Fixation was stopped by adding glycine to a final concentration of 125mM. Fixed cells were lysed and sonicated. DNA was immunoprecipitated, eluted, de-crosslinked, treated with RNase and protease, and purified. Library was constructed using Illumina ChIP-Seq DNA sample prep kit and sequencing was done on Illumina GAII.

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

hg38

Number of total reads

26065791

Reads aligned (%)

96.2

Duplicates removed (%)

12.7

Number of peaks

986 (qval < 1E-05)

hg19

Number of total reads

26065791

Reads aligned (%)

95.4

Duplicates removed (%)

13.9

Number of peaks

1128 (qval < 1E-05)