Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BCOR

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
Cultured Human Embryonic Stem Cells
cell type
H1 hESC
genotype
WT
media
mTeSR (StemCell Technolgies)
media supplement
NA
antibody
Vivian Bardwell Lab; University of Minnesota, Cat# anti-BCOR, RRID:AB_2716801
fixation strategy
formaldehyde
sonication strategy
Covaris S220

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNAeasy Micro Kit (Qiagen) was used to prepare RNA from the samples described above. TruSeq (Illumina) RNA libraries were prepared from the recovered RNA.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
26091650
Reads aligned (%)
93.1
Duplicates removed (%)
4.6
Number of peaks
12765 (qval < 1E-05)

hg38

Number of total reads
26091650
Reads aligned (%)
95.0
Duplicates removed (%)
3.3
Number of peaks
13050 (qval < 1E-05)

Base call quality data from DBCLS SRA