Bisulfite-Seq analysis of WGBS Lib 70 derived from human fMuscle Trunk cells
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Bisulfite-Seq
Antigen
Bisulfite-Seq
Cell type
Cell type Class
Muscle
Cell type
Fetal muscle
NA
NA
Attributes by original data submitter
Sample
COLLECTION_METHOD
Post-Mortem
DONOR_HEALTH_STATUS
presumed normal
MOLECULE
genomic DNA
DISEASE
presumed normal
TISSUE_TYPE
Fetal Muscle, Trunk
DONOR_ETHNICITY
Unknown
DONOR_SEX
Female
BIOMATERIAL_TYPE
Primary Tissue
TISSUE_DEPOT
Trunk
DONOR_ID
UW H24720
BIOMATERIAL_PROVIDER
University of Washington
DONOR_AGE
115.0 Days
BioSampleModel
Generic
sample_term_id
UBERON_0001774
Sequenced DNA Library
library_name
WGBS_Lib 70
library_strategy
Bisulfite-Seq
library_source
GENOMIC
library_selection
RANDOM
library_construction_protocol
Genomic DNA (1.5~5µg) was fragmented to 100-500 bp using a Covaris S2. Purified DNA fragments were end-repaired. After A-tailing, the DNA fragments were ligated with methylated paired-end adapters. Adapter-attached DNA fragments of 300-400 bp, which contain 150-250 bp genomic DNA inserts, were gel-purified. The purified DNA fragments were subjected to two cycles of sodium bisulfite conversion using the EpiTect Bisulfite kit (Qiagen). Adapter-attached, bisulfite-converted DNA molecules were enriched by PCR using PfuTurboCx Hotstart DNA polymerase (Stratagene). The PCR amplified DNA fragments were subjected to a second gel size selection to remove PCR primers and adapter dimers. The enriched library was quantified using a Qubit fluorometer and Quant-iT dsDNA HS Assay Kit. Library sequencing was performed using 101 BP PE Illumina technology.