Bisulfite-Seq analysis of WGBS Lib 62 derived from human adult CD14 cells
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Bisulfite-Seq
Antigen
Bisulfite-Seq
Cell type
Cell type Class
Blood
Cell type
CD14+ cells
NA
NA
Attributes by original data submitter
Sample
DONOR_HEALTH_STATUS
presumed normal
MOLECULE
genomic DNA
DISEASE
presumed normal
MARKERS
CD14+
PASSAGE_IF_EXPANDED
NA
CELL_TYPE
adult CD14
DONOR_ETHNICITY
Unknown
DONOR_SEX
Male
BIOMATERIAL_TYPE
Primary Cell
DONOR_ID
UW RO 02035
BIOMATERIAL_PROVIDER
UW
DONOR_AGE
37.0 Years
BioSampleModel
Generic
sample_term_id
CL_0001054
Sequenced DNA Library
library_name
WGBS_Lib 62
library_strategy
Bisulfite-Seq
library_source
GENOMIC
library_selection
RANDOM
library_construction_protocol
Genomic DNA (1.5~5µg) was fragmented to 100-500 bp using a Covaris S2. Purified DNA fragments were end-repaired. After A-tailing, the DNA fragments were ligated with methylated paired-end adapters. Adapter-attached DNA fragments of 300-400 bp, which contain 150-250 bp genomic DNA inserts, were gel-purified. The purified DNA fragments were subjected to two cycles of sodium bisulfite conversion using the EpiTect Bisulfite kit (Qiagen). Adapter-attached, bisulfite-converted DNA molecules were enriched by PCR using PfuTurboCx Hotstart DNA polymerase (Stratagene). The PCR amplified DNA fragments were subjected to a second gel size selection to remove PCR primers and adapter dimers. The enriched library was quantified using a Qubit fluorometer and Quant-iT dsDNA HS Assay Kit. Library sequencing was performed using 101 BP PE Illumina technology.