Cells grown on 10-cm dishes were fixed with 1% formaldehyde-containing medium for 10 min and were quenched by glycine. Collected samples were then subjected to ChIP assay using anti-H3K4me1 (MBL, MABI0302), anti-H3K4me3 (MBL, MABI0304), anti-H3K27me3 (MBL, MABI0323), anti-H3K27ac (MBL, MABI0309S) antibodies. Sequencing libraries were made using the NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina