Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

immortalized mouse embryonic fibroblast cell line

cell line

R26M2rtTA/+;TetOcre;Sdhcfl/wt

day

16

antibody

anti-H3K4me3 (Abcam cat# ab8580, lot# GR273043-4)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells harvested by trypsinization and fixed with formaldehyde prior to lysis. Chromatin prepared via MNase digestion and sonication. Either ChIP-enriched chromatin or non-enriched input chromatin used for library construction. ThruPLEX DNA-seq 48D Kit (Rubicon Genomics cat# R400406)

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

38926605

Reads aligned (%)

97.9

Duplicates removed (%)

18.1

Number of peaks

1768 (qval < 1E-05)

mm9

Number of total reads

38926605

Reads aligned (%)

97.7

Duplicates removed (%)

18.6

Number of peaks

1771 (qval < 1E-05)