Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Muscle

Cell type

Tibialis anterior muscle

NA

NA

Sample

source_name

tibialis anterior muscle

tissue

tibialis anterior muscle

timepoint

14 days

chip antibody

H3 K27ac (Active Motif 39133)

treatment

freeze injury

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Frozen tissue thawed, homogenized, cells lysed Chromatin sheared and incubated overnight at 4C with antibodies using constant agitation; co-immunoprecipitated

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

23333273

Reads aligned (%)

95.4

Duplicates removed (%)

74.8

Number of peaks

771 (qval < 1E-05)

mm9

Number of total reads

23333273

Reads aligned (%)

95.3

Duplicates removed (%)

75.0

Number of peaks

796 (qval < 1E-05)