Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Muscle

Cell type

Tibialis anterior muscle

NA

NA

Sample

source_name

tibialis anterior muscle

tissue

tibialis anterior muscle

timepoint

10 hours

chip antibody

H3 K4me3 (Millipore 07-743, lots 2178350 & 2289139)

treatment

freeze injury

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Frozen tissue thawed, homogenized, cells lysed Chromatin sheared and incubated overnight at 4C with antibodies using constant agitation; co-immunoprecipitated

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

21836997

Reads aligned (%)

86.8

Duplicates removed (%)

25.6

Number of peaks

4908 (qval < 1E-05)

mm9

Number of total reads

21836997

Reads aligned (%)

86.5

Duplicates removed (%)

25.7

Number of peaks

4814 (qval < 1E-05)